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申峰, 张杰, 艾明珠, 张越东, 刘赵淼. 微流控粒子分选中圆形微凹槽容纳特性研究[J]. 力学学报.
引用本文: 申峰, 张杰, 艾明珠, 张越东, 刘赵淼. 微流控粒子分选中圆形微凹槽容纳特性研究[J]. 力学学报.
Study on the Holding Characteristics of Round Microcavities in Microfluidic Particle Sorting[J]. Chinese Journal of Theoretical and Applied Mechanics.
Citation: Study on the Holding Characteristics of Round Microcavities in Microfluidic Particle Sorting[J].Chinese Journal of Theoretical and Applied Mechanics.

微流控粒子分选中圆形微凹槽容纳特性研究

Study on the Holding Characteristics of Round Microcavities in Microfluidic Particle Sorting

  • 摘要:微流控技术(Microfluidics)为粒子和细胞操控提供了新平台。基于粒子惯性迁移和微凹槽涡胞捕获的粒子分选方法,成为一种重要的微流控粒子操控技术。目前,微凹槽容纳的捕获粒子数量不高,制约了该方法的效率。为提高微凹槽粒子容量,对圆形微凹槽进行结构设计,并利用高速显微成像技术和数值模拟,研究了不同圆形微凹槽的粒子容纳能力。研究发现,相同入口雷诺数(Re = 37~555)下,带底腔的圆形微凹槽相较于普通圆形微凹槽容纳的粒子数量提升了45%;当Re = 444时,带底腔的直径500 μm圆凹槽比直径600 μm圆凹槽的粒子容量提高了54.7%,原因是前者凹槽内内粒子运动轨道与流线更加吻合,粒子轨道面积与凹槽面积占比达到了97%;从侧通道收集到的20 μm粒子的最大富集浓度为初始悬浮液的126.7倍。研究结果对微凹槽结构设计、提高粒子分选性能有重要指导意义。

    Abstract:Microfluidics provides a new platform for the manipulation of particles and cells. The particle sorting method based on particle inertial migration and vortex trapping in microcavities has become an important microfluidic particle manipulation technique. Currently, the number of captured particles accommodated by the microcavity is not high, which restricts the sorting efficiency of this method. To improve the holding capacity, different round microcavities were designed, and their holding characteristics for sorted particles were investigated by using high-speed microimaging technology and numerical simulation. The results show that under the same inlet Reynolds number (Re=37~555), the number of captured particles in a round microcavity with a bottom chamber can be enhanced by 45% compared with that in an ordinary round microcavity. At Re=444, the holding capacity in a round cavity with a diameter of 500 μm increases by 54.7% compared to that with a diameter of 600 μm. The reason is that the particle motion track in the former cavity is more consistent with the streamlines, and the particle trajectory area and cavity area ratio researches 97%. The maximum concentration of 20-μm particles collected from the side channel is 126.7 times higher than that of the initial suspension. The results could provide useful guidance for the microcavity design and improvement of particle sorting performance.

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